Document Type : Original Article
Authors
1
Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Assiut University, Assiut, Egypt
2
Department of Oral Pathology, Faculty of Dentistry, Al-Azhar University (Assiut Branch), Assiut, Egypt
3
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Al-Azhar University (Assiut Branch), Assiut, Egypt
4
Department of Oral Medicine, Periodontology, Oral Diagnosis and Dental Radiology, Faculty of Dentistry, Al-Azhar University (Assiut Branch), Assiut, Egypt
5
Department of Oral Medicine, Oral Diagnosis and Periodontology, Faculty of Oral and Dental Medicine, South Valley University, Qena, Egypt
6
Department of Oral Pathology, Faculty of Dentistry, Alexandria University, Alexandria, Egypt
Abstract
Introduction: Oral cancer is one of the critical global social health troubles. Lymph nodes metastasis of oral carcinomas have
a significant prognosis impact. Authentic TNM staging of the neoplasms is essential to bear an assign board for disease control
and prognostication. The model of grading histologically from only tissue biopsy has limitations. Abnormal DNA content has
been correlated with malignant diseases. Flow cytometry can be a rapid method for quantitative test of DNA in solid tumors.
Prediction the metastatic deposit in relation to the DNA flow cytometry inquiry is the point of importance.
Aim of the study: This study was operated to matched between tumor DNA content to the clinical stages of oral squamous cell
carcinoma patients; to better predict the tumor behavior and to guide treatment planning.
Material and Method: A total of 40 fresh/frozen biopsies in different TNM clinical stages of oral cancer were divided into 2
groups. Group I; twenty lesions classified in stage I and stage II. Group II; twenty tumors classified as stage III and stage IV. All
specimens utilized to DNA flow cytometric scanning in order to achieve the DNA ploidy and S-phase fraction.
Results: The difference in aneuploidy state and S-phase fraction between Group I, and Group II was highly statistical significant
in relation to their clinical stages on the basis of clinical information and biopsy results.
Conclusions: DNA flow cytometric analysis of oral carcinomas excisions reported a significant predication to a late metastatic
clinical stages of oral cancer lesions.
Keywords
)
View on SCiNiTO