Biomimetic effect of a natural leaf extract in clavarial defects (Experimental study)

Document Type : Original Article


1 Oral and Maxillofacial Surgery Department, Faculty of Oral and Dental Medicine, Badr University, Egypt

2 Oral and Maxillofacial Pathology Department, Faculty of Dentistry, Cairo University, Egypt

3 Oral and Maxillofacial Surgery Department, Faculty of Dentistry, Cairo University, Egypt


Defects in Oral and Maxillofacial region constitutes a challenge for surgeons to reconstruct. Invention of different strategies
involving new biomaterials that hasten the natural regenerative steps is a persistent need. Natural sources gained attention in
the past few years.
Objective: Evaluate the effectiveness of Moringa Oleifera leaf (MOL) powder and extract mixture locally and MO leaf extract
repeated injections in critical size clavarial defects in rabbits.
Materials and Methods: Moringa oleifera fresh leaf powder and aqueous extract were prepared. Eight new zealand rabbits
were included in this study; two animals were used as control. The rest six were used as intervention groups. All animals
were subjected to creation of bilateral clavarial defect in the parietal bone, both sides of the test animals were treated with
a mixture of Moringa Oleifera leaf powder (0.35 gm) and water extract (½ml). The right side was additionally injected with
Moringa Oleifera leaf water extract (¼ ml) twice (3 days a part postoperatively). The specimens were sent for histopathologic
examination by H&E and Masson Trichrome stain to quantify new bone formation, defect narrowness.
Results: UMoringa Oleifera leaf (MOL) extract repeated injections sites showed more newly formed bone, fewer inflammatory
cells and narrower bone defects.
Conclusion: Moringa Oleifera leave Polyphenolic extracts: Gallic, Chlorogenic acids, and Rutin affected bone healing
parameters positively.