Enhancement role of Adipose Derived Stem Cells Vs Platelet Rich Fibrin in an induced bony defect

Document Type : Original Article

Authors

1 Department of Oral Biology, Faculty of Dentistry, The Egyptian Russian University, Cairo

2 Department of Oral and maxillofacial surgery, Hospital, Banha University Hospitals, Benha, Egypt.

3 Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine Benha University.

4 Genetics and genetic engineering, Department of animal wealth development, Faculty of Veterinary Medicine, Benha University.

Abstract

Autologous Adipose derived stem cells (ADSCs) are seen to be safe and abundant source of mesenchymal stem cells. Platelet
rich fibrin (PRF) is platelet distillate that used usually in dental surgery setting, it contains many growth factors that help in
alveolar bone healing. The experiment aimed to compare the bone formation in bone defects on either side of the maxillae
of 2 dogs, after application of ADSCs (on the left side) and PRF (on the right side). Each defect was labelled as a group, as
follows: group 1 and group 2 designated for the right sides, treated with PRF on Gelfoam®, of the brown dog and the black
dog respectively, groups 3 and 4 were for the brown and black dogs respectively, and they were treated with the ADCSCs on a
Gelfoam. After six months, the bone formation in the four groups was assessed using Computed Tomography (CT), and level
of gene expression of Aggrecan, and collagen type two using PCR. CT indicated an increase in bone formation in the left sides
treated with ADSCs in comparison with the right sides supplemented with PRF. The same goes for gene expression of Collagen
type 2 and Aggrecan genes. We concluded that ADSCs generated more mineralized tissue than PRF over six months’ period.

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History

  • Receive Date: 06 February 2022
  • Revise Date: 17 February 2022
  • Accept Date: 02 June 2022

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