Enhancement role of Adipose Derived Stem Cells Vs Platelet Rich Fibrin in an induced bony defect

Document Type : Original Article


1 Egyptian Russian University

2 Department of Oral and maxillofacial surgery, Hospital, Banha University Hospitals, Benha, Egypt.

3 Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine Benha University. Benha, Egypt

4 Genetics and genetic engineering, Department of animal wealth development, Faculty of Veterinary Medicine, Benha University.


Autologous Adipose derived stem cells (ADSCs) are seen to be safe and abundant source of mesenchymal stem cells. Platelet rich fibrin (PRF) is platelet distillate that used usually in dental surgery setting, it contains many growth factors that help in alveolar bone healing. The experiment aimed to compare the bone formation in bone defects on either side of the maxillae of 2 dogs, after application of ADSCs (on the left side) and PRF (on the right side). Each defect was labelled as a group, as follows: group 1 and group 2 designated for the right sides, treated with PRF on Gelfoam®, of the brown dog and the black dog respectively, groups 3 and 4 were for the brown and black dogs respectively, and they were treated with the ADCSCs on a Gelfoam. After six months, the bone formation in the four groups was assessed using Computed Tomography (CT), and level of gene expression of Aggrecan, and collagen type two using PCR. CT indicated an increase in bone formation in the left sides treated with ADSCs in comparison with the right sides supplemented with PRF. The same goes for gene expression of Collagen type 2 and Aggrecan genes. We concluded that ADSCs generated more mineralized tissue than PRF over six months’ period.


Articles in Press, Accepted Manuscript
Available Online from 18 August 2022
  • Receive Date: 06 February 2022
  • Revise Date: 01 April 2023
  • Accept Date: 27 June 2022
  • First Publish Date: 18 August 2022